Bit9 raises $54.5 million in its biggest round yet

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Bit9 Inc., a Waltham cybersecurity firm, has raised $54.5 million in its biggest funding round to date.

The company, which has done business under the name Bit9+Carbon Black since acquiring Carbon Black last year, sells cybersecurity software to businesses. It said it would use the new funds to fuel its “explosive growth” and expansion in other countries.

Several major venture investors took part in the company’s series F funding round, including the Boston firms .406 Ventures, Accomplice, and Highland Capital Partners and the Silicon Valley firms Kleiner Perkins Caufield & Byers and Sequoia Capital. Two new companies, Founders Circle and Evolution Equity Partners, also took part in the round.

The company said the latest round boosted its total funds raised to $175 million.

Bit9 also said it would reach several significant milestones by the end of 2015, hitting $70 million in revenue, increasing its revenue by 70 percent, selling more than 7 million software licenses, and hitting nearly 2,000 customers. The company declined to disclose whether it was profitable. It has around 475 employees, with roughly 300 in Waltham, a spokesman said.